Nucleosomes at Active Promoters: Unforgettable Loss
نویسندگان
چکیده
منابع مشابه
Nucleosomes at active promoters: unforgettable loss.
A variety of chromatin features have been implicated in the regulation of gene expression, including nucleosome occupancy at promoters, histone modifications and variants, and DNA methylation. In this issue of Cancer Cell, Lin and colleagues use a powerful single-molecule approach to explore the relationship between nucleosome occupancy and gene expression in cancer cells. They show that nucleo...
متن کاملNucleosomes unfold completely at a transcriptionally active promoter.
It has long been known that promoter DNA is converted to a nuclease-sensitive state upon transcriptional activation. Recent findings have raised the possibility that this conversion reflects only a partial unfolding or other perturbation of nucleosomal structure, rather than the loss of nucleosomes. We report topological, sedimentation, nuclease digestion, and ChIP analyses, which demonstrate t...
متن کاملChromatin remodelers clear nucleosomes from intrinsically unfavorable sites to establish nucleosome-depleted regions at promoters
Most promoters in yeast contain a nucleosome-depleted region (NDR), but the mechanisms by which NDRs are established and maintained in vivo are currently unclear. We have examined how genome-wide nucleosome placement is altered in the absence of two distinct types of nucleosome remodeling activity. In mutants of both SNF2, which encodes the ATPase component of the Swi/Snf remodeling complex, an...
متن کاملAsymmetric nucleosomes flank promoters in the budding yeast genome.
Nucleosomes in active chromatin are dynamic, but whether they have distinct structural conformations is unknown. To identify nucleosomes with alternative structures genome-wide, we used H4S47C-anchored cleavage mapping, which revealed that 5% of budding yeast (Saccharomyces cerevisiae) nucleosome positions have asymmetric histone-DNA interactions. These asymmetric interactions are enriched at n...
متن کاملAnthracyclines induce double-strand DNA breaks at active gene promoters.
Doxorubicin is a widely used chemotherapeutic drug that intercalates between DNA base-pairs and poisons Topoisomerase II, although the mechanistic basis for cell killing remains speculative. Doxorubicin and related anthracycline compounds have been shown to increase nucleosome turnover and/or eviction around promoters, which suggests that the resulting enhanced exposure of DNA might underlie ce...
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ژورنال
عنوان ژورنال: Cancer Cell
سال: 2007
ISSN: 1535-6108
DOI: 10.1016/j.ccr.2007.10.024